Here's a thread about a few guys trying to get more ethanol into their fuel by mixing E10 (the gas that is in most pumps nowadays) and E85.
I will try to summarize, but I am not the most knowledgeable so please correct me if I summarized wrong.
Too much ETHANOL in a car that is not designed for it CAN and WILL DESTROY your fuel system, a GM engineer at Nationals told me NOT to put ANY amount of E85 in the car because things WILL break.GM Flex Fuel vehicles are meant to handle high ethanol levels and have some fancy gear in them to optimize for that fuel. I'm sure the other manufacturers do this as well.
That being said... here you go.
The experiment was begun by I Am Broke on HPTuners forum. He is a very experienced tuner and allegedly knows a lot about fuels, I think he works with fuel for his job, and I don't mean pumping it.
He's able to increase timing a lot because ethanol has a higher octane than gasoline. But ethanol doesn't have as much energy in it as gas, so you can reach a point where you start to lose performance.
Right now 1 gallon of E85 to 1 gallon of 93 octane E10 gave him the best performance. He mentions 60-100 times
Too much ethanol and you need more fuel pressure from the HPFP so he got a Trifecta tune, but the additional pressure didn't make it better. So he went back to 1:1 and has settled there.
Here is the thread. Lots of info there.
http://www.hptuners.com/forum/showthread.php?t=30353